SanDiegoRecruiter Since 2001
the smart solution for San Diego jobs

Research Assistant II - Molecular & Cell Biology Laboratory, Dr. Diana Hargreaves

Company: Salk Institute for Biological Studies
Location: La Jolla
Posted on: January 14, 2021

Job Description:

Application Instructions: Employment History Include your last ten (10) years of employment history, or length of your employment history if less, including all position (even those that are not relevant to this position) and periods of unemployment. Incomplete information could disqualify you from further consideration. POSITION SUMMARY: Position available in the Hargreaves Lab for overall lab management duties in support of the Principal Investigator’s (PI) studies. General lab maintenance duties include maintaining adequate inventory of materials and equipment to ensure adequate supplies for experimental needs, ordering supplies, taking inventory, upkeep and safety. Position is also responsible for performing various molecular biology, biochemistry, and cell biology techniques in support of lab and PI research goals. Range of duties include, but is not limited to: conducting procedures, modifying procedures as needed, data collection and analysis, tissue culture, recombinant DNA techniques and laboratory maintenance. ESSENTIAL FUNCTIONS (50%) Molecular Biology Techniques Perform chromatin immunoprecipitation, RNA purification, next-generation library preparation, ATAC-seq, CUT&RUN, CUT&TAG, and RT-qPCR for the study of epigenetic regulators. Immunoprecipitation, Western blotting, gel electrophoresis, small and large scale DNA purification, cloning, and cell staining. Molecular cloning to mutate genes and express mutated genes in cells, for example using CRIPSR/Cas 9 engineering. (40%) Tissue Culture Culturing of various mammalian cell types including primary mouse cells and human cancer cell lines. Production of lentiviruses. Perform transfections and lentiviral infection for overexpression/knockdown in various cell types. Isolation of protein, DNA, and RNA from cells. Perform immunofluorescence (10%) Lab Support Order general lab supplies and prepare stocks and reagents for common use. Manage and perform maintenance of mouse colony including setting up breeders. Manage lab equipment. Attend lab meetings and assist in general problem solving. Prepare PowerPoint slides detailing results for presentation. Train new lab members. EDUCATION Required: Bachelor’s degree of science in Biology/Chemistry and/or related field. EXPERIENCE Required: Minimum 2 years’ experience with laboratory techniques. Preferred: Previous experience as a lab technician or equivalent Experience with next-generation sequencing library preparation a plus SKILLS AND ABILITIES Required: Theoretical and hands on knowledge of cell maintenance and molecular biology techniques. Theoretical knowledge of biological sciences; genetic linkage analysis methods; Mendelian genetics and molecular biological techniques, including the polymerase chain reaction (PCR). Demonstrated knowledge and experience in researching information and analyzing data to arrive at valid conclusions. Skill in the operation and maintenance of various lab equipment. Ability to independently design experiments and debug protocols. Ability to manage and track large numbers of samples meticulously without mixup or cross contamination. Knowledge of standard laboratory management and safety procedures. Ability to make independent decisions, analyze situations, and solve problems. Analytical and critical thinking skills to identify and communicate problems. Proven ability to work both independently and also as part of a collaborative team. Excellent communication skills, both oral and written. Ability to communicate and act as a liaison with a diverse audience of professionals. Strong interpersonal skills, including tact, diplomacy, and flexibility. Ability to establish and maintain effective working relationships; interact with all levels of management and employees across multiple departments. SPECIAL CONDITIONS OF EMPLOYMENT Must be willing to work in a mammalian cell biology-related research environment. Must be willing to work an adjusted schedule to support research needs. Must be willing to work in an animal-related research environment. Must be willing to sign a confidentiality agreement. Successful completion of the Institute’s background investigation. PHYSICAL REQUIREMENTS This position requires constant adjusting focus, grasping, hearing, keying, seeing, sitting, talking, analyzing, calculating, communicating, reading, reasoning, writing, and working inside.

Keywords: Salk Institute for Biological Studies, San Diego , Research Assistant II - Molecular & Cell Biology Laboratory, Dr. Diana Hargreaves, Healthcare , La Jolla, California

Click here to apply!

Didn't find what you're looking for? Search again!

I'm looking for
in category
within


Other Healthcare Jobs


Travel Nurse RN - Skilled Nursing Facility - $2,015 per week
Description: TotalMed Staffing is seeking a travel nurse RN Skilled Nursing Facility for a travel nursing job in Poway, California.Job Description RequirementsSpecialty: Skilled Nursing FacilityDiscipline: RNStart (more...)
Company: NurseFly
Location: Poway
Posted on: 01/18/2021

Travel Nurse RN - Long Term Care - $2,159 per week
Description: Stability Healthcare is seeking a travel nurse RN Long Term Care for a travel nursing job in Poway, California.Job Description Requirements ul li Specialty: Long Term Care li Discipline: RN li (more...)
Company: NurseFly
Location: Poway
Posted on: 01/18/2021

Travel Nurse RN - Rehabilitation - $2,165 per week
Description: Stability Healthcare is seeking a travel nurse RN Rehabilitation for a travel nursing job in Poway, California.Job Description Requirements ul li Specialty: Rehabilitation li Discipline: RN li (more...)
Company: NurseFly
Location: Poway
Posted on: 01/18/2021


Part-Time Charge Nurse (LPN)
Description: Job Description The Palms at La Quinta, the premier assisted living community
Company: Hawthorn Senior Living
Location: La Quinta
Posted on: 01/18/2021

Travel Nurse RN - ED - Emergency Department - $2,875 per week
Description: Stability Healthcare is seeking a travel nurse RN ED - Emergency Department for a travel nursing job in Poway, California.Job Description Requirements ul li Specialty: ED - Emergency Department (more...)
Company: NurseFly
Location: Poway
Posted on: 01/18/2021

School Speech Language Pathologist - Make up to $2,040.00 take home per week
Description: Job Description Were looking for an exceptional Bilingual School Speech Language
Company: TherapyTravelers
Location: Santee
Posted on: 01/18/2021

In Home Caregiver - Flexible Scheduling
Description: No need to job search any further, we have the caregiver position that you've been looking for Apply now and you can start within the week Full-time or part-time, morning, afternoon, or evening shifts (more...)
Company: CareInHomes
Location: Santee
Posted on: 01/18/2021

Travel RN-PCU
Description: Telemetry opening in Placentia, California. This and other rn jobs brought to you by NursingJobCafe.com Specialty: RN-PCU -TravelLocations: Placentia, CaliforniaShift: Days Duration: 13 weeksStart Date: (more...)
Company: All Medical Personnel
Location: Placentia
Posted on: 01/18/2021

Intensive Care Nurse
Description: Job Description RequirementsIntensive Care Nurse - ICU Nurse - ICU RN StartDate: ASAP Available Shifts: 12 D/E/N Pay Rate: 1401.00 - 1545.00This position presents an opportunity to join an elite team (more...)
Company: American Mobile Healthcare
Location: Poway
Posted on: 01/18/2021

Travel Nurse RN - Med Surg - $2,659 per week
Description: Stability Healthcare is seeking a travel nurse RN Med Surg for a travel nursing job in Poway, California.Job Description Requirements ul li Specialty: Med Surg li Discipline: RN li Start Date: (more...)
Company: NurseFly
Location: Poway
Posted on: 01/18/2021

Log In or Create An Account

Get the latest California jobs by following @recnetCA on Twitter!

San Diego RSS job feeds