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Staff Research Assistant 1

Company: University of California - San Diego Medical Cente
Location: San Diego
Posted on: December 5, 2019

Job Description:

This is a 100% limited appointment for 10 months with the possibility of extension for an additional 2 months, depending on project needs and funding availability. UCSD Layoff from Career Appointment: Apply by 11/20/2019 for consideration with preference for rehire. All layoff applicants should contact their Employment Advisor. Special Selection Applicants: Apply by 12/2/2019. Eligible Special Selection clients should contact their Disability Counselor for assistance. DESCRIPTION =========== The Staff Research Assistant 1 will perform biochemical and genetic studies of novel filaments in the budding yeast, S. cerevisiae. Responsibilities include a wide variety of standard repetitive procedures such as: generating particular yeast via genetic techniques and analyzing the progeny by a combination of live imaging, immunoblotting, enzymatic assays, immunofluorescence and other techniques. The incumbent will also be responsible for the following: generating recombinant DNA constructs that the individual and other members of the laboratory will use for protein expression as well as for making yeast strains; the expression and purification of filament forming proteins and the development of assays for polymerization; and the maintenance and feeding of the yeast stocks for the laboratory. Laboratory administration/management duties include: ordering supplies, lab organization, maintenance of computer and laboratory equipment, and providing work direction to undergraduate students. * Must be willing and able to be flexible with work hours to include occasional evening or weekend work as required by particular experiments. QUALIFICATIONS ============== * Theoretical and practical knowledge of biochemistry, molecular biology, immunology and genetics, including relevant laboratory techniques such as genomic technologies using RNAi and microarray analyses, acquired through a Bachelor's Degree in Biology or a related field, and/or an equivalent combination of education and laboratory experience. * Theoretical knowledge and previous experience with a variety of small- and large-scale molecular biology techniques, including: DNA preparation, PCR, in vitro transcription, nucleic acid purification, enzyme restriction digests, gel electrophoresis, ligation, transformation, screening for clones, plasmid construction, isolation and analysis, preparation of radioactive probe for hybridization and autoradiagraphy, sequencing, microarray hybridizations, protein purification, immunofluorescence, and microinjection. * Previous experience with S. cerevisiae yeast and familiarity with genetic crosses. * Proven ability to execute multi-step assays and multi-generational yeast crosses. * Demonstrated knowledge of cell and protein analyses, including experience with Western blot analysis and subcellular localization by fluorescent microscopy. * Proven ability to express and purify proteins using affinity and conventional chromatography * Proven ability to develop enzyme assays for protein activity including assays for the ability to self-assemble. * Working knowledge to operate various lab instruments including: bright-field and fluorescent microscopes, centrifuges, spectrophotometer, hemocytometer, micropipetting equipment, and computer for maintaining stock inventories and protocols. * Skill, knowledge and ability to provide the full range of supervisory duties (select, train, work-direction, and disciplinary action up to and including release) for undergraduate student lab employees. * Demonstrated knowledge and proven ability to order lab supplies and equipment, and to proactively and appropriate facilitate the maintenance of all lab supplies and equipment. * Familiar with PC computers and software such as Microsoft Office, with skill in use of PC computer databases for quality control, tracking, and data input. Willingness and ability to learn new hardware and software as needed. * Demonstrated knowledge of and experience with Material Safety Data Sheets (MSDS's) and hazard communication and Injury and Illness Prevention Programs. * Familiarity and experience with a Chemical Hygiene Plan addressing safety precautions including appropriate clothing and shoes, safe handling of chemicals, and use of protective items such as gloves and goggles. Proven ability and willingness to keep safety training current and consistent with research involvement. * Skill, ability, and willingness to learn new laboratory techniques, lab equipment, and computer hardware/software rapidly and as needed. * Proven ability and willingness to read scientific papers as directed in order to stay abreast of developments within the field and relating to current experiments. * Excellent verbal and written communication skills with the ability to maintain clear, concise lab notes and to communicate experimental results effectively on the telephone or via email. Positive and motivated attitude, and the ability to work congenially and collaboratively with others in growing lab environment. * Proven ability to work independently with supervision in efficient and organized manner. * Ability to embrace and consistently uphold the UC San Diego Principles of Community for a collaborative work environment. SPECIAL CONDITIONS ================== * Must be willing to work with radioisotopes.

Keywords: University of California - San Diego Medical Cente, San Diego , Staff Research Assistant 1, Other , San Diego, California

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