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Staff Research Associate III

Company: University of California - San Diego Medical Cente
Location: San Diego
Posted on: November 22, 2020

Job Description:

This is a UC San Diego internal recruitment open to UCSD and UCSD Health System staff only. UCSD Layoff from Career Appointment: Apply by 10/30/20 for consideration with preference for rehire. All layoff applicants should contact their Employment Advisor. Special Selection Applicants: Apply by 11/03/20. Eligible Special Selection clients should contact their Disability Counselor for assistance. DESCRIPTION =========== UCSD Skaggs School of Pharmacy and Pharmaceutical Sciences is growing in the diverse areas of professional educational programs, cutting-edge research, and pharmacy services. Research areas specific to this position posting are related to medication safety in the ambulatory care setting, practice-based research, collaborative practice, and a pharmacogenomics education program. The school is committed to offering a dynamic learning environment and growing opportunities to its talented and dedicated employees. Under general supervision, the Staff Research Associate III will independently design and implement experiments primarily related to expression, purification and characterization of purified human acetylcholinesterase (hAChE). Expression will be performed in standard mammalian cell lines and incumbent will also be responsible for maintaining the tissue culture (TC) facility including basic TC assistance to other lab members and training of new TC users. The incumbent will be responsible for implementing and performing a variety of new and on-going experimental protocols, training new lab members on all aspects of the protein expression, purification and characterization, and making significant research contributions to the laboratory. Along with the chemical and cellular biological research, the incumbent will independently analyze and present experimental data to lab and external collaborators. Incumbent will also serve as Area Safety Coordinator for the laboratory responsible for oversight of proper application of environmental safety procedures that will include chemical hazards, biological hazards and radioactive isotope inventory and radioactive hazard handling. The incumbent will be responsible for EH&S training administration and book keeping for all lab members the supervision of the isotope laboratory, maintenance of inventory records and oversight of wipe testing to maintain the laboratory's Biological Use Authorization (BUA)radioactive license (RUA) as well as assist in general laboratory maintenance and organization, which includes supply maintenance, inventory, ordering and preparation for planned experiments. MINIMUM QUALIFICATIONS ====================== * Advanced theoretical knowledge of molecular biology, chemistry and biochemistry as typically attained through a college degree, or an equivalent combination of education and laboratory experience. * Proven ability to perform biochemical assays and laboratory procedures, including preparation of buffers and reagents. Ability to monitor viability of cell lines, freeze, thaw and culture using sterile techniques. Advanced knowledge of bacteriology, plasmid DNA amplification, and molecular biology techniques. * Extensive experience in a research laboratory, including laboratory organization and management. * Strong skills in supervising laboratory staff and providing advanced training in the use of laboratory equipment and analytical software/tools. Experience facilitating organization and efficiency in a fast-paced, complex laboratory environment. * Proven experience with the use of general laboratory equipment including centrifuges, microscopes, liquid nitrogen cryotanks, spectrophotometer, microtiter plate readers. Experience and proficiency in mammalian tissue culture protein expression and purification assays. Additionally molecular and cellular biology techniques including but not limited to DNA and RNA purification and analysis. * Proven skills necessary to purify enzymes from over-expressing mammalian cells by affinity chromatography. * Demonstrated skill in purifying plasmid DNA for transfections. * Excellent computer and data management skills. Proven expertise in a variety of general programs, such as Microsoft Office Suite and use of scientific software for data analysis. * Demonstrated skill and experience in writing and editing material for grants, reports, and peer-reviewed scholarly publications. * Proven ability to research, design and implement original, effective strategies for coordinating and managing projects on a daily basis. Demonstrated experience and proficiency in protocol design and implementation. * Demonstrated experience in making significant contributions to research designs of various ongoing research projects. Demonstrated skill in problem solving and critical thinking. PREFERRED QUALIFICATIONS ======================== * Knowledge of UC and EH&S laboratory safety procedures (particularly concerning radioactive material). * Knowledge of UC procedures for ordering, purchasing, shipping and inventory of supplies and equipment. SPECIAL CONDITIONS ================== * Employment is subject to a criminal background check. * Willingness to work evenings and weekends, if necessary. * Must be willing to work with hazardous materials.

Keywords: University of California - San Diego Medical Cente, San Diego , Staff Research Associate III, Other , San Diego, California

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